An Essential Role for TAGLN2 in Phagocytosis of Lipopolysaccharide-activated Macrophages.
Sci Rep
; 7(1): 8731, 2017 08 18.
Article
em En
| MEDLINE
| ID: mdl-28821818
ABSTRACT
Activated macrophages have a greater ability of phagocytosis against pathogens that is mediated by large-scale actin rearrangement. However, molecular machineries that conduct this task have not been fully identified. Here, we demonstrate an unanticipated role of TAGLN2, a 22-kDa actin-binding protein, in Toll-like receptor (TLR)-stimulated phagocytosis. TAGLN2 was greatly induced in macrophages in response to lipopolysaccharide (LPS), a ligand for TLR4, partly via the NF-κB pathway. TAGLN2-deficient macrophages (TAGLN2 -/-) showed defective phagocytic functions of IgM- and IgG-coated sheep red blood cells as well as bacteria. Cell signaling pathways involved in actin rearrangement-PI3 kinase/AKT and Ras-ERK-were also down-regulated in LPS-stimulated TAGLN2-deficient macrophages. Moreover, TAGLN2 -/- mice showed higher mortality after bacterial infection than wild-type littermates. Thus, our results revealed a novel function of TAGLN2 as a molecular armament required for host defense.
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Base de dados:
MEDLINE
Assunto principal:
Fagocitose
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Lipopolissacarídeos
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Macrófagos Peritoneais
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Ativação de Macrófagos
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Proteínas dos Microfilamentos
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Proteínas Musculares
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article