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An optimised protocol for isolation of RNA from small sections of laser-capture microdissected FFPE tissue amenable for next-generation sequencing.
Amini, Parisa; Ettlin, Julia; Opitz, Lennart; Clementi, Elena; Malbon, Alexandra; Markkanen, Enni.
Afiliação
  • Amini P; Institute of Veterinary Pharmacology and Toxicology, Vetsuisse Faculty, University of Zürich, Winterthurerstr. 260, 8057, Zurich, Switzerland.
  • Ettlin J; Institute of Veterinary Pharmacology and Toxicology, Vetsuisse Faculty, University of Zürich, Winterthurerstr. 260, 8057, Zurich, Switzerland.
  • Opitz L; Functional Genomics Center Zurich, University of Zürich/ETH Zürich, Winterthurerstr. 190, 8057, Zurich, Switzerland.
  • Clementi E; Institute of Veterinary Pharmacology and Toxicology, Vetsuisse Faculty, University of Zürich, Winterthurerstr. 260, 8057, Zurich, Switzerland.
  • Malbon A; Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zürich, Winterthurerstr. 268, 8057, Zurich, Switzerland.
  • Markkanen E; Institute of Veterinary Pharmacology and Toxicology, Vetsuisse Faculty, University of Zürich, Winterthurerstr. 260, 8057, Zurich, Switzerland. enni.markkanen@vetpharm.uzh.ch.
BMC Mol Biol ; 18(1): 22, 2017 08 23.
Article em En | MEDLINE | ID: mdl-28835206
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA / Análise de Sequência de RNA / Sequenciamento de Nucleotídeos em Larga Escala / Microdissecção e Captura a Laser / Biologia Molecular Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA / Análise de Sequência de RNA / Sequenciamento de Nucleotídeos em Larga Escala / Microdissecção e Captura a Laser / Biologia Molecular Idioma: En Ano de publicação: 2017 Tipo de documento: Article