In vivo photothermal optical coherence tomography of endogenous and exogenous contrast agents in the eye.
Sci Rep
; 7(1): 9228, 2017 08 23.
Article
em En
| MEDLINE
| ID: mdl-28835698
ABSTRACT
Optical coherence tomography (OCT) has become a standard-of-care in retinal imaging. OCT allows non-invasive imaging of the tissue structure but lacks specificity to contrast agents that could be used for in vivo molecular imaging. Photothermal OCT (PT-OCT) is a functional OCT-based technique that has been developed to detect absorbers in a sample. We demonstrate in vivo PT-OCT in the eye for the first time on both endogenous (melanin) and exogenous (gold nanorods) absorbers. Pigmented mice and albino mice (n = 6 eyes) were used to isolate the photothermal signal from the melanin in the retina. Pigmented mice with laser-induced choroidal neovascularization lesions (n = 7 eyes) were also imaged after a systemic injection of gold nanorods to observe their passive accumulation in the retina. This experiment demonstrates the feasibility of PT-OCT to image the distribution of both endogenous and exogenous absorbers in the mouse retina.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Retina
/
Meios de Contraste
/
Tomografia de Coerência Óptica
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article