Purification of biomevalonate from fermentation broth and conversion of biomevalonate into biomevalonolactone.
J Biotechnol
; 259: 46-49, 2017 Oct 10.
Article
em En
| MEDLINE
| ID: mdl-28842180
ABSTRACT
Mevalonate (MVA) is a key compound of living organisms including bacteria, plants, and humans. MVA and mevalonolactone (MVL), a lactonized form of MVA, are important for pharmaceutical, cosmeceutical, and biotechnological applications. Although (R, S)-MVA with 50% enantiomeric purity is mainly produced by chemical synthesis, recently, microbial fermentation processes for MVA production have been considered as an alternative to the chemical synthesis because of high enantiomeric purity [(R)-MVA] and high titer. In the present study, bio-MVA produced by a fermentative process was decolorized by a charcoal-based method and then chemically transformed into bio-MVL without byproducts by means of phosphoric acid as an acid catalyst. The final bio-MVL was (R)-MVL with over 99% enantiomeric purity according to 1H NMR analysis.
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Base de dados:
MEDLINE
Assunto principal:
Ácido Mevalônico
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article