Expression and immunological evaluation of elongation factor Tu of Streptococcus suis serotype 2.
Pol J Vet Sci
; 20(2): 277-284, 2017 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-28865219
ABSTRACT
Streptococcus suis serotype 2 (SS2) is considered as a major pathogen that causes sepsis and meningitis in piglets and humans, but knowledge of its antigenic proteins remains limited so far. The surface-related proteins of pathogens often play significant roles in bacterium-host interactions and infection. Here, we obtained the elongation factor Tu (EF-Tu) gene of Streptococcus suis and constructed the recombinant expression plasmid successfully. The target recombinant plasmid was then expressed in Escherichia coli and the immuno-protection of the recombinant protein was subsequently evaluated as well. The EF-Tu gene of Streptococcus suis is 1197 bp in length, encodes 398 amino acids. The target recombinant EF-Tu (rEF-Tu) protein can recognize the antiserum of Streptococcus suis and can provoke obvious humoral immune responses in rabbits and conferred protection to rabbits against Streptococcus suis ear-vein challenge, implying that the EF-Tu may be used as an attractive candidate antigen for a component of subunit vaccine.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
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Regulação Bacteriana da Expressão Gênica
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Fator Tu de Elongação de Peptídeos
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Streptococcus suis
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article