Poly-ϵ-caprolactone/chitosan nanoparticles provide strong adjuvant effect for hepatitis B antigen.
Nanomedicine (Lond)
; 12(19): 2335-2348, 2017 Oct.
Article
em En
| MEDLINE
| ID: mdl-28868964
ABSTRACT
AIM:
This work aims to investigate the adjuvant effect of poly-ϵ-caprolactone/chitosan nanoparticles (NPs) for hepatitis B surface antigen (HBsAg) and the plasmid DNA encoding HBsAg (pRC/CMV-HBs).METHODS:
Both antigens were adsorbed onto preformed NPs. Vaccination studies were performed in C57BL/6 mice. Transfection efficiency was investigated in A549 cell line.RESULTS:
HBsAg-adsorbed NPs generated strong anti-HBsAg IgG titers, mainly of IgG1 isotype, and induced antigen-specific IFN-γ and IL-17 secretion by spleen cells. The addition of pRC/CMV-HBs to the HBsAg-adsorbed NPs inhibited IL-17 secretion but had minor effect on IFN-γ levels. Lastly, pRC/CMV-HBs-loaded NPs generated a weak serum antibody response.CONCLUSION:
Poly-ϵ-caprolactone/chitosan NPs provide a strong humoral adjuvant effect for HBsAg and induce a Th1/Th17-mediated cellular immune responses worth explore for hepatitis B virus vaccination.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Poliésteres
/
Adjuvantes Imunológicos
/
Quitosana
/
Nanopartículas
/
Antígenos de Superfície da Hepatite B
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article