TREK-1 mediates isoflurane-induced cytotoxicity in astrocytes.
BMC Anesthesiol
; 17(1): 124, 2017 Sep 05.
Article
em En
| MEDLINE
| ID: mdl-28870160
ABSTRACT
BACKGROUND:
There are growing concerns that anaesthetic exposure can cause extensive apoptotic degeneration of neurons and the impairment of normal synaptic development and remodelling. However, little attention has been paid to exploring the possible cytotoxicity of inhalation anaesthetics, such as isoflurane, in astrocytes. In this research, we determined that prolonged exposure to an inhalation anaesthetic caused cytotoxicity in astrocytes, and we identified the underlying molecular mechanism responsible for this process.METHODS:
Astrocytes were exposed to isoflurane, and astrocytic survival was then measured via LDH release assays, MTT assays, and TUNEL staining. TWIK-related potassium (K+) channel-1 (TREK-1) over-expression and knockdown models were also created using lentiviruses. The levels of TREK-1 and brain-derived neurotrophic factor (BDNF) were measured via Western blot and qRT-PCR.RESULTS:
Prolonged exposure to isoflurane decreased primary astrocytic viability in a dose- and time-dependent manner. Moreover, with prolonged exposure to isoflurane, the TREK-1 level increased, and the BDNF level was reduced. TREK-1 knockdown promoted the survival of astrocytes and increased BDNF expression following isoflurane exposure.CONCLUSIONS:
Overdoses of and prolonged exposure to isoflurane induce cytotoxicity in primary astrocytes. TREK-1 plays an important role in isoflurane-induced cultured astrocytic cytotoxicity by down-regulating the expression of BDNF.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Astrócitos
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Anestésicos Inalatórios
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Canais de Potássio de Domínios Poros em Tandem
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Citotoxinas
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Isoflurano
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article