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SERPING1 mRNA overexpression in monocytes from HIV+ patients.
Sanfilippo, C; Cambria, D; Longo, A; Palumbo, M; Avola, R; Pinzone, M; Nunnari, G; Condorelli, F; Musumeci, G; Imbesi, R; Castogiovanni, P; Malaguarnera, L; Di Rosa, Michelino.
Afiliação
  • Sanfilippo C; Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy.
  • Cambria D; Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy.
  • Longo A; Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy.
  • Palumbo M; Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy.
  • Avola R; Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy.
  • Pinzone M; Department of Pathology and Laboratory Medicine, School of Medicine, University of Pennsylvania, Philadelphia, USA.
  • Nunnari G; Unit of Infectious Diseases, Department of Clinical and Experimental Medicine, University of Messina, Messina, Italy.
  • Condorelli F; Department of Pharmacological Sciences, Università del Piemonte Orientale "A. Avogadro", 28100, Novara, Italy.
  • Musumeci G; Human Anatomy and Histology Section, Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.
  • Imbesi R; Human Anatomy and Histology Section, Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.
  • Castogiovanni P; Human Anatomy and Histology Section, Department of Biomedical and Biotechnological Sciences, School of Medicine, University of Catania, Catania, Italy.
  • Malaguarnera L; Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy.
  • Di Rosa M; Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy. mdirosa@unict.it.
Inflamm Res ; 66(12): 1107-1116, 2017 Dec.
Article em En | MEDLINE | ID: mdl-28889214
OBJECTIVE: The HIV-1 virus activates the complement system, an essential element of the immune system. SERPING1 is a protease inhibitor that disables C1r/C1s in the C1 complex of the classical complement pathway. METHODS: In this paper, we performed an analysis of several microarrays deposited in GEO dataset to demonstrate that SERPING1 mRNA is modulated in CD14+ monocytes from HIV-1-infected individuals. In addition, data were validated on monocytes isolated from seronegative healthy volunteers, treated with IFNs. RESULTS: Our analysis shows that SERPING1 mRNA is overexpressed in monocytes from HIV-1+ patients and the expression levels correlate positively with viral load and negatively with the CD4+ T-cell count. Of note, anti-retroviral therapy is able to reduce the levels of SERPING1 mRNA, ex vivo. In addition, we found that 30% of the SERPING1 genes network is upregulated in monocytes from HIV-1+ patients. Noteworthy, the expression levels of IFITM1-an antiviral molecule belonging to the genes network-correlate positively with SERPING1 expression. Interestingly, the monocytes treatment with IFN-gamma, IFN-beta and IFN-alpha significantly upregulates the SERPING1 mRNA expression levels. CONCLUSIONS: From the outcome of our investigation, it is possible to conclude that SERPING1 and its network serve as important components of the innate immune system to restrict HIV-1 infection.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Monócitos / Infecções por HIV / Proteína Inibidora do Complemento C1 Idioma: En Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA Mensageiro / Monócitos / Infecções por HIV / Proteína Inibidora do Complemento C1 Idioma: En Ano de publicação: 2017 Tipo de documento: Article