A Mass Spectrometry-Based Approach for Mapping Protein Subcellular Localization Reveals the Spatial Proteome of Mouse Primary Neurons.

Itzhak, Daniel N; Davies, Colin; Tyanova, Stefka; Mishra, Archana; Williamson, James; Antrobus, Robin; Cox, Jürgen; Weekes, Michael P; Borner, Georg H H

Itzhak, Daniel N; Davies, Colin; Tyanova, Stefka; Mishra, Archana; Williamson, James; Antrobus, Robin; Cox, Jürgen; Weekes, Michael P; Borner, Georg H H.

Afiliação

Itzhak DN; Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany.
Davies C; Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK.
Tyanova S; Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany.
Mishra A; Department of Molecules-Signaling-Development, Max Planck Institute of Neurobiology, 82152 Martinsried, Germany.
Williamson J; Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK.
Antrobus R; Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK.
Cox J; Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany.
Weekes MP; Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK.
Borner GHH; Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany. Electronic address: borner@biochem.mpg.de.

Cell Rep ; 20(11): 2706-2718, 2017 Sep 12.

Article em En | MEDLINE | ID: mdl-28903049

Assuntos

Neurônios/metabolismo; Proteoma/metabolismo; Proteômica/métodos; Espectrometria de Massas em Tandem/métodos; Animais; Biomarcadores/metabolismo; Fracionamento Celular; Células Cultivadas; Células HeLa; Humanos; Marcação por Isótopo; Camundongos; Organelas/metabolismo; Transporte Proteico; Coloração e Rotulagem; Frações Subcelulares/metabolismo

Palavras-chave

EGF signaling; LFQ; TMT; label-free quantification; neurons; organellar proteomics; primary cells; quantitative mass spectrometry; spatial proteomics; tandem mass tagging

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteoma / Proteômica / Espectrometria de Massas em Tandem / Neurônios Idioma: En Ano de publicação: 2017 Tipo de documento: Article

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