DNA aptamer generation by ExSELEX using genetic alphabet expansion with a mini-hairpin DNA stabilization method.
Biochimie
; 145: 15-21, 2018 Feb.
Article
em En
| MEDLINE
| ID: mdl-28916151
ABSTRACT
A novel aptamer generation method to greatly augment the affinity and stability of DNA aptamers was developed by genetic alphabet expansion combined with mini-hairpin DNA technology. The genetic alphabet expansion increases the physicochemical and structural diversities of DNA aptamers by introducing extra components, unnatural bases, as a fifth base, allowing for the enhancement of DNA aptamer affinities. Furthermore, the mini-hairpin DNA technology stabilizes DNA aptamers against nuclease digestion and thermal denaturation, by introducing an extraordinarily stable mini-hairpin DNA containing a GCGAAGC sequence. This novel method provides stabilized high-affinity DNA aptamers for diagnostic and therapeutic applications.
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Base de dados:
MEDLINE
Assunto principal:
Aptâmeros de Nucleotídeos
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Técnica de Seleção de Aptâmeros
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Conformação de Ácido Nucleico
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article