[Effect of protein intervention on amino acid metabolism spectrum of Qi and Yin deficiency type 2 diabetic rats].
Zhongguo Zhong Yao Za Zhi
; 41(22): 4226-4233, 2016 Nov.
Article
em Zh
| MEDLINE
| ID: mdl-28933093
ABSTRACT
To study the effect of plant protein and animal protein on amino acid metabolism spectrum of Qi and Yin deficiency type 2 diabetic rats. 110 male SD rats were randomly divided into blank group (n=10), diabetic model group (n=20), disease-symptoms group (n=80). The rats of blank group received ordinary feeding, while other groups were fed with high sugar and fat diets. During the whole process of feeding, rats of disease-symptoms group were given with Qingpi-Fuzi (15.75 gâ¢kg⻹) once a day through oral administration. Five weeks later, the rats were given with a low dose of STZ (40 mgâ¢kg⻹) by intraperitoneal injection to establish experimental diabetic models. Then the models were randomly divided into disease-symptoms group 1 (Qi and Yin deficiency diabetic group, 15.75 gâ¢kg⻹), disease-symptoms group 2 (plant protein group, 0.5 gâ¢kg⻹), disease-symptoms group 3 (animal protein group, 0.5 gâ¢kg⻹), disease-symptoms group 4 (berberine group, 0.1 gâ¢kg⻹). The drugs were given for 4 weeks by gavage administration. After 4 weeks of protein intervention, the abdominal aortic blood was collected and serum was isolated to analyze its free amino acid by using AQC pre-column derivatization HPLC and fluorescence detector. Four weeks after the protein intervention, plant protein, animal protein and berberine had no obvious effect on body weight and blood sugar in type 2 diabetic rats. As compared with animal protein group, histidine and prolineï¼P<0.01ï¼, serine, glycine, threonine, alanine, tyrosine, valine, methionine, bright+isoleucine, phenylalanine and lysineï¼P<0.05ï¼changed a lot in rats serum of plant protein group.The results showed that gavage administration of protein would produce effects on amino acid metabolism of Qi and Yin deficiency type 2 diabetic SD rats. Symbolic differential compounds could be found through metabonomics technology, providing experimental basis for early warning of type 2 diabetes and diagnosis of Qi and Yin deficiency syndrome.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Medicamentos de Ervas Chinesas
/
Deficiência da Energia Yin
/
Diabetes Mellitus Experimental
/
Dieta
/
Aminoácidos
Idioma:
Zh
Ano de publicação:
2016
Tipo de documento:
Article