Profiling of miRNAs in porcine germ cells during spermatogenesis.
Reproduction
; 154(6): 789-798, 2017 12.
Article
em En
| MEDLINE
| ID: mdl-28947561
ABSTRACT
Spermatogenesis includes mitosis of spermatogonia, meiosis of pachytene spermatocytes and spermiogenesis of round spermatids. MiRNAs as a ~22 nt small noncoding RNA are involved in regulating spermatogenesis at post-transcriptional level. However, the dynamic miRNAs expression in the developmental porcine male germ cells remains largely undefined. In this study, we purified porcine spermatogonia, pachytene spermatocytes and round spermatids using a STA-PUT apparatus. A small RNA deep sequencing and analysis were conducted to establish a miRNAs profiling in these male germ cells. We found that 19 miRNAs were differentially expressed between spermatogonia and pachytene spermatocytes, and 74 miRNAs differentially expressed between pachytene spermatocytes and round spermatids. Furthermore, 91 miRNAs were upregulated, while 108 miRNAs were downregulated in spermatozoa. We demonstrated that ssc-miR-10a-5p, ssc-miR-125b, ssc-let-7f and ssc-miR-186 were highly expressed in spermatogonia, pachytene spermatocytes, round spermatids and spermatozoa respectively. The findings could provide novel insights into roles of miRNAs in regulation of porcine spermatogenesis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Espermatogênese
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Espermatozoides
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Perfilação da Expressão Gênica
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MicroRNAs
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Sequenciamento de Nucleotídeos em Larga Escala
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article