[Cloning and protein expression analysis of geranyl diphosphate synthase genes in Tripterygium wilfordii].
Zhongguo Zhong Yao Za Zhi
; 42(2): 220-225, 2017 Jan.
Article
em Zh
| MEDLINE
| ID: mdl-28948723
ABSTRACT
Based on the transcriptome data, the study cloned full-length cDNA of TwGPPS1 and TwGPPS2 genes from Tripterygium wilfordii suspension cells and then analyzed the bioinformation of the sequence and protein expression. The cloned TwGPPS1 has a 1 278 bp open reading frame (ORF) encoding a polypeptide of 425 amino acids. The deduced isoelectric point (pI) was 6.68, a calculated molecular weight was about 47.189 kDa. The full-length cDNA of the TwGPPS2 contains a 1 269 bp open reading frame (ORF) encoding a polypeptide of 422 amino acids. The deduced isoelectric point (pI) was 6.71, a calculated molecular weight was about 46.774 kDa.The entire reading frame of TwGPPS1,2 was cloned into the pET-32a(+) vector and expressed in E. coli BL21 (DE3) cells to obtain the TwGPPS protein, which laid a basis for further study on the regulation of terpenoid secondary metabolism and biological synthesis.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
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Difosfatos
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Tripterygium
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Diterpenos
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Geraniltranstransferase
Idioma:
Zh
Ano de publicação:
2017
Tipo de documento:
Article