Quantitation Method for Polyfunctional Thiols in Hops (Humulus lupulus L.) and Beer Using Specific Extraction of Thiols and Gas Chromatography-Tandem Mass Spectrometry.
Anal Chem
; 89(21): 11598-11604, 2017 11 07.
Article
em En
| MEDLINE
| ID: mdl-28972741
ABSTRACT
A method for the quantitation of six polyfunctional thiols, 4-methyl-4-sulfanylpentan-2-one (4MSP), 3-sulfanyl-4-methylpentan-1-ol (3S4MP), 3-sulfanyl-4-methylpentyl acetate (3S4MPA), 3-sulfanyl-3-methylbutan-1-ol (3S3MB), 3-sulfanylhexan-1-ol (3SH), and 3-sulfanylhexyl acetate (3SHA), in hops and beer without organic mercury compounds was developed. The method employed specific extraction of thiols using a silver ion solid phase extraction (SPE) cartridge and gas chromatography-tandem mass spectrometry (GC-MS/MS). For all thiols analyzed, good linearity was achieved by adding thioglycerol as an analyte protectant. Recoveries for both hops (74-100%) and beer (79-113%) were acceptable, and the repeatability for both was also good (relative standard deviations of 2.8-8.4%). The limits of detection for the six polyfunctional thiols were below their odor thresholds in beer. The method was applied to quantitation of hops and beer flavored with thiol-containing hop varieties. Due to their detected levels and level variations in different beers, 4MSP and 3S4MP are thought to be important polyfunctional thiols for the characteristic flavor of hop varieties.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Compostos de Sulfidrila
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Cerveja
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Humulus
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Espectrometria de Massas em Tandem
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Extração em Fase Sólida
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Cromatografia Gasosa-Espectrometria de Massas
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article