Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides.
Chem Commun (Camb)
; 53(88): 11992-11995, 2017 Nov 14.
Article
em En
| MEDLINE
| ID: mdl-28984884
ABSTRACT
The 10DM24 deoxyribozyme can site-specifically label RNAs with fluorophore-GTP conjugates; however, the 2',5'-branched RNA linkage is readily cleaved by debranchase. To prevent loss of labels upon cleavage, we synthesized phosphorothioate-modified, fluorescent GTP derivatives and elaborated conditions for their incorporation by 10DM24. RNAs labeled with fluorescent derivatives of Sp-GTPS were found to be resistant to debranchase.
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Base de dados:
MEDLINE
Assunto principal:
Coloração e Rotulagem
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RNA
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DNA Catalítico
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Corantes Fluorescentes
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Guanosina Trifosfato
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article