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Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides.
Carrocci, Tucker J; Lohe, Lea; Ashton, Matthew J; Höbartner, Claudia; Hoskins, Aaron A.
Afiliação
  • Carrocci TJ; Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Dr., Madison, WI 53706, USA. ahoskins@wisc.edu.
  • Lohe L; Institute for Organic and Biomolecular Chemistry, Georg-August-University Göttingen, Tammannstr. 2, 37077 Göttingen, Germany.
  • Ashton MJ; Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Dr., Madison, WI 53706, USA. ahoskins@wisc.edu.
  • Höbartner C; Institute for Organic and Biomolecular Chemistry, Georg-August-University Göttingen, Tammannstr. 2, 37077 Göttingen, Germany and Institute for Organic Chemistry, Julius-Maximilians-University Würzburg, Am Hubland, 97074 Würzburg, Germany. claudia.hoebartner@uni-wuerzburg.de.
  • Hoskins AA; Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Dr., Madison, WI 53706, USA. ahoskins@wisc.edu.
Chem Commun (Camb) ; 53(88): 11992-11995, 2017 Nov 14.
Article em En | MEDLINE | ID: mdl-28984884
ABSTRACT
The 10DM24 deoxyribozyme can site-specifically label RNAs with fluorophore-GTP conjugates; however, the 2',5'-branched RNA linkage is readily cleaved by debranchase. To prevent loss of labels upon cleavage, we synthesized phosphorothioate-modified, fluorescent GTP derivatives and elaborated conditions for their incorporation by 10DM24. RNAs labeled with fluorescent derivatives of Sp-GTPS were found to be resistant to debranchase.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Coloração e Rotulagem / RNA / DNA Catalítico / Corantes Fluorescentes / Guanosina Trifosfato Idioma: En Ano de publicação: 2017 Tipo de documento: Article
Texto completo
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XML
PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Coloração e Rotulagem / RNA / DNA Catalítico / Corantes Fluorescentes / Guanosina Trifosfato Idioma: En Ano de publicação: 2017 Tipo de documento: Article