CXCL12-CXCR4 signalling plays an essential role in proper patterning of aortic arch and pulmonary arteries.
Cardiovasc Res
; 113(13): 1677-1687, 2017 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-29016745
ABSTRACT
AIMS:
Chemokine CXCL12 (stromal derived factor 1 SDF1) has been shown to play important roles in various processes of cardiovascular development. In recent avian studies, CXCL12 signalling has been implicated in guidance of cardiac neural crest cells for their participation in the development of outflow tract and cardiac septum. The goal of this study is to investigate the extent to which CXCL12 signalling contribute to the development of aortic arch and pulmonary arteries in mammals. METHODS ANDRESULTS:
Novel Cxcl12-LacZ reporter and conditional alleles were generated. Using whole mount X-gal staining with the reporter allele and vascular casting techniques, we show that the domain branching pattern of pulmonary arteries in Cxcl12-null mice is completely disrupted and discordant with that of pulmonary veins and airways. Cxcl12-null mice also displayed abnormal and superfluous arterial branches from the aortic arch. The early steps of pharyngeal arch remodelling in Cxcl12-null mice appeared to be unaffected, but vertebral arteries were often missing and prominent aberrant arteries were present parallel to carotid arteries or trachea, similar to aberrant vertebral artery or thyroid ima artery, respectively. Analysis with computed tomography not only confirmed the results from vascular casting studies but also identified abnormal systemic arterial supply to lungs in the Cxcl12-null mice. Tie2-Cre mediated Cxcr4 deletion phenocopied the Cxcl12-null phenotypes, indicating that CXCR4 is the primary receptor for arterial patterning, whereas Cxcl12 or Cxcr4 deletion by Wnt1-Cre did not affect aortic arch patterning.CONCLUSION:
CXCL12-CXCR4 signalling is essential for the correct patterning of aortic arches and pulmonary arteries during development. Superfluous arteries in Cxcl12-null lungs and the aortic arch infer a role of CXCL12 in protecting arteries from uncontrolled sprouting during development of the arterial system.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Aorta Torácica
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Artéria Pulmonar
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Padronização Corporal
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Receptores CXCR4
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Malformações Vasculares
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Quimiocina CXCL12
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article