Comparative analysis of ginsenosides in human glucocorticoid receptor binding, transactivation, and transrepression.
Eur J Pharmacol
; 815: 501-511, 2017 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-29031898
ABSTRACT
Conflicting data exist on the effect of ginsenosides on transactivation of human glucocorticoid receptor α (herein referred to as glucocorticoid receptor), and relatively little is known regarding the effect of these chemicals on transrepression of this receptor. We investigated the effect of 20(S)-protopanaxadiol (PPD), PPD-type ginsenosides (Rb1, Rb2, Rc, Rd, Rh2, and Compound K), 20(S)-protopanaxatriol (PPT), and PPT-type ginsenosides (Re, Rf, Rg1, and Rh1) on glucocorticoid receptor binding, transactivation, and transrepression. Each ginsenoside was less efficacious than dexamethasone (positive control) in binding to the ligand-binding domain of glucocorticoid receptor. Among the ginsenosides investigated, Rh2 had the smallest IC50 value (15 ± 1µM), whereas it was 0.02 ± 0.01µM for dexamethasone. In contrast to dexamethasone, none of the ginsenosides influenced glucocorticoid receptor transactivation or transrepression in LS180 human colorectal adenocarcinoma cells, as assessed in a dual-luciferase reporter gene assay. Rh2 did not affect the endogenous mRNA level of tyrosine aminotransferase (marker for glucocorticoid receptor transactivation) or corticosteroid-binding globulin (marker for glucocorticoid receptor transrepression) in HepG2 human hepatocellular carcinoma cells. This chemical also did not alter the response by a glucocorticoid receptor agonist (dexamethasone or Compound A) in the dual-luciferase reporter gene assay or target gene expression assay. In conclusion, ginsenosides were less efficacious and less potent than dexamethasone in binding to the ligand-binding domain of glucocorticoid receptor. The number of glycosylated groups was associated with a decrease in receptor binding potency. PPD-type and PPT-type ginsenosides are not modulators of glucocorticoid receptor transactivation or transrepression in LS180 and HepG2 cells.
Palavras-chave
20(S)-Protopanaxadiol (PubChem CID: 11213350); 20(S)-Protopanaxatriol (PubChem CID: 11468733); Compound A (PubChem CID: 9838147); Dexamethasone (PubChem CID: 5743); Ginsenoside; Ginsenoside Compound K (PubChem CID: 9852086); Ginsenoside Rb1 (PubChem CID: 9898279); Ginsenoside Rb2 (PubChem CID: 6917976); Ginsenoside Rc (PubChem CID: 12855889); Ginsenoside Rd (PubChem CID: 11679800); Ginsenoside Rh2 (PubChem CID: 119307); Glucocorticoid receptor; Protopanaxadiol; Protopanaxatriol; Transactivation; Transrepression
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Receptores de Glucocorticoides
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Ativação Transcricional
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Ginsenosídeos
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article