Analysis of Borrelia burgdorferi Proteome and Protein-Protein Interactions.

Yang, Xiuli; Thakur, Meghna; Koci, Juraj; Smith, Alexis A; Singh, Preeti; Zhuang, Xuran; Promnares, Kamoltip; Wang, Yan; Buyuktanir, Ozlem; Pal, Utpal

Yang, Xiuli; Thakur, Meghna; Koci, Juraj; Smith, Alexis A; Singh, Preeti; Zhuang, Xuran; Promnares, Kamoltip; Wang, Yan; Buyuktanir, Ozlem; Pal, Utpal.

Afiliação

Yang X; Department of Veterinary Medicine, University of Maryland, College Park, MD, USA.
Thakur M; Department of Veterinary Medicine, University of Maryland, College Park, MD, USA.
Koci J; Department of Veterinary Medicine, University of Maryland, College Park, MD, USA.
Smith AA; Department of Veterinary Medicine, University of Maryland, College Park, MD, USA.
Singh P; Department of Veterinary Medicine, University of Maryland, College Park, MD, USA.
Zhuang X; Department of Veterinary Medicine, University of Maryland, College Park, MD, USA.
Promnares K; Department of Veterinary Medicine, University of Maryland, College Park, MD, USA.
Wang Y; Proteomics Core Facility, College of Computer, Mathematical, and Natural Sciences, University of Maryland, College Park, MD, 20742, USA.
Buyuktanir O; Department of Microbiology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey. ozlemb@omu.edu.tr.
Pal U; Department of Veterinary Medicine, University of Maryland, College Park, MD, USA.

Methods Mol Biol ; 1690: 259-277, 2018.

Article em En | MEDLINE | ID: mdl-29032550

Assuntos

Proteínas de Bactérias/metabolismo; Borrelia burgdorferi/metabolismo; Doença de Lyme/microbiologia; Mapeamento de Interação de Proteínas/métodos; Mapas de Interação de Proteínas; Proteômica/métodos; Proteínas de Bactérias/análise; Far-Western Blotting/métodos; Borrelia burgdorferi/química; Eletroforese em Gel Bidimensional/métodos; Eletroforese em Gel de Poliacrilamida/métodos; Células HEK293; Humanos; Espectrometria de Massas/métodos; Proteoma/análise; Proteoma/metabolismo; Técnicas do Sistema de Duplo-Híbrido

Palavras-chave

Borrelia burgdorferi; Far-Western; Luciferase assay; Mass spectrometry; Proteinprotein interaction; Proteomics analysis; Two-dimensional (2-D) gel electrophoresis; Yeast two-hybrid

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Doença de Lyme / Borrelia burgdorferi / Mapeamento de Interação de Proteínas / Proteômica / Mapas de Interação de Proteínas Idioma: En Ano de publicação: 2018 Tipo de documento: Article

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