Dynamics of lineage commitment revealed by single-cell transcriptomics of differentiating embryonic stem cells.
Nat Commun
; 8(1): 1096, 2017 10 23.
Article
em En
| MEDLINE
| ID: mdl-29061959
ABSTRACT
Gene expression heterogeneity in the pluripotent state of mouse embryonic stem cells (mESCs) has been increasingly well-characterized. In contrast, exit from pluripotency and lineage commitment have not been studied systematically at the single-cell level. Here we measure the gene expression dynamics of retinoic acid driven mESC differentiation from pluripotency to lineage commitment, using an unbiased single-cell transcriptomics approach. We find that the exit from pluripotency marks the start of a lineage transition as well as a transient phase of increased susceptibility to lineage specifying signals. Our study reveals several transcriptional signatures of this phase, including a sharp increase of gene expression variability and sequential expression of two classes of transcriptional regulators. In summary, we provide a comprehensive analysis of the exit from pluripotency and lineage commitment at the single cell level, a potential stepping stone to improved lineage manipulation through timing of differentiation cues.
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Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Embrionárias
/
Análise de Célula Única
/
Transcriptoma
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article