Follicle dynamics: visualization and analysis of follicle growth and maturation using murine ovarian tissue culture.
J Assist Reprod Genet
; 35(2): 339-343, 2018 Feb.
Article
em En
| MEDLINE
| ID: mdl-29080194
ABSTRACT
PURPOSE:
To visualize and analyze follicle development in ovarian tissue culture using physiological concentrations of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in order to establish an ovarian tissue culture system that enables efficient in vitro growth of follicles.METHODS:
Ovarian tissues from 4-week-old female ICR mice were sliced and cultured. Images of ovarian tissues in culture were obtained at 24-h or 30-min intervals by using a microscope. The area of each follicle observed in the ovarian tissue slices was tracked and analyzed in association with oocyte maturation.RESULTS:
We were able to track the development of each follicle using this culture system. Follicle growth was associated with oocyte maturation. Meiotically matured oocytes (MII) were obtained from 33% of all follicles investigated. Approximately, a quarter of follicles (24%) did not grow and resulted in atresia.CONCLUSION:
Follicle dynamics were successfully visualized and analyzed in murine ovarian tissue culture. We were able to obtain mature oocytes from the fully grown follicles in vitro. This culture system would be helpful for efficient in vitro culturing of ovarian tissues.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Ovário
/
Técnicas de Cultura de Tecidos
/
Folículo Ovariano
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article