Preparation of an Efficient Ratiometric Fluorescent Nanoprobe (m-CDs@[Ru(bpy)3]2+) for Visual and Specific Detection of Hypochlorite on Site and in Living Cells.
ACS Sens
; 2(11): 1684-1691, 2017 11 22.
Article
em En
| MEDLINE
| ID: mdl-29094932
ABSTRACT
Hypochlorite (ClO-) is one of the most important reactive oxygen species (ROS), which plays an important role in sustaining human innate immunity during microbial invasion. Moreover, ClO- is a powerful oxidizer for water treatment. The safety of drinking water is closely related to its content. Herein, m-phenylenediamine (mPD) is used as a precursor to prepare carbon dots (named m-CDs) with highly fluorescent quantum yield (31.58% in water), and our investigation shows that the strong fluorescent emission of m-CDs can be effectively quenched by ClO-. Based on these findings, we developed a novel fluorescent nanoprobe (m-CDs) for highly selective detection of ClO-. The linear range was from 0.05 to 7 µM (R2 = 0.998), and the limit of detection (S/N = 3) was as low as 0.012 µM. Moreover, a portable agarose hydrogel solid matrix-based ratiometric fluorescent nanoprobe (m-CDs@[Ru(bpy)3]2+) sensor was subsequently developed for visual on-site detection of ClO- with the naked eyes under a UV lamp, suggesting its potential in practical application with low cost and excellent performance in water quality monitoring. Additionally, intracellular detection of exogenous ClO- was demonstrated via ratiometric imaging microscopy.
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Base de dados:
MEDLINE
Assunto principal:
Compostos Organometálicos
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Carbono
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Ácido Hipocloroso
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Nanotecnologia
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Nanoestruturas
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Nanopartículas
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Corantes Fluorescentes
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article