A monoclonal antibody raised against a thermo-stabilised ß<sub>1</sub>-adrenoceptor interacts with extracellular loop 2 and acts as a negative allosteric modulator of a sub-set of ß<sub>1</sub>-adrenoceptors expressed in stable cell lines.

Soave, Mark; Cseke, Gabriella; Hutchings, Catherine J; Brown, Alastair J H; Woolard, Jeanette; Hill, Stephen J

A monoclonal antibody raised against a thermo-stabilised ß₁-adrenoceptor interacts with extracellular loop 2 and acts as a negative allosteric modulator of a sub-set of ß₁-adrenoceptors expressed in stable cell lines.

Soave, Mark; Cseke, Gabriella; Hutchings, Catherine J; Brown, Alastair J H; Woolard, Jeanette; Hill, Stephen J.

Afiliação

Soave M; Division of Physiology, Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK; Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham and University of Nottingham, Midlands, UK.
Cseke G; Heptares Therapeutics Ltd., Bio Park, Welwyn Garden City AL7 3AX, UK.
Hutchings CJ; Heptares Therapeutics Ltd., Bio Park, Welwyn Garden City AL7 3AX, UK.
Brown AJH; Heptares Therapeutics Ltd., Bio Park, Welwyn Garden City AL7 3AX, UK.
Woolard J; Division of Physiology, Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK; Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham and University of Nottingham, Midlands, UK. Electronic address: jeanette.woolard@nottingham.
Hill SJ; Division of Physiology, Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK; Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham and University of Nottingham, Midlands, UK. Electronic address: stephen.hill@nottingham.ac.u

Biochem Pharmacol ; 147: 38-54, 2018 01.

Article em En | MEDLINE | ID: mdl-29102678

Assuntos

Anticorpos Monoclonais/química; Anticorpos Monoclonais/metabolismo; Receptores Adrenérgicos beta 1/química; Receptores Adrenérgicos beta 1/metabolismo; Agonistas Adrenérgicos beta/metabolismo; Regulação Alostérica/fisiologia; Sequência de Aminoácidos; Animais; Anticorpos Monoclonais/genética; Células CHO; Cricetinae; Cricetulus; Células HEK293; Humanos; Propanolaminas/metabolismo; Ligação Proteica/fisiologia; Estabilidade Proteica; Estrutura Secundária de Proteína; Receptores Adrenérgicos beta 1/genética; Turquia

Palavras-chave

Allosterism; Alprenolol (Pubchem CID: 2119); CGP 12177 (Pubchem CID: 2687); CGP 20712A (Pubchem CID: 2685); Cimaterol (Pubchem CID: 2755); Extracellular loop 2; Furimazine (Pubchem CID: 219083); GPCR; Hoechst 33342 (Pubchem CID: 1464); IBMX (Pubchem CID: 3758); ICI 118551 (Pubchem CID: 5484725); Isoprenaline (Pubchem CID: 5807); Monoclonal antibody; Propranolol (Pubchem CID: 4946)

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Receptores Adrenérgicos beta 1 / Anticorpos Monoclonais Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar no Google