Protein extracted from Porphyra yezoensis prevents cisplatin-induced nephrotoxicity by downregulating the MAPK and NF-κB pathways.
Int J Mol Med
; 41(1): 511-520, 2018 Jan.
Article
em En
| MEDLINE
| ID: mdl-29115386
ABSTRACT
Acute renal failure is a serious complication of treatment with the anticancer drug cisplatin. Cisplatin exerts a cytotoxic effect on renal cells by inducing apoptosis through activating the tumor suppressor p53, nuclear factorκB (NFκB) and mitogenactivated protein kinase (MAPK)/p38 pathways. Effects of protein extracts of the brown seaweed Porphyra yezoensis (P. yezoensis) on cytotoxicity, inflammation and cell proliferation have been reported; however, the effects of P. yezoensis protein (PYP) extract on cisplatininduced renal injury have remained elusive. The present study investigated the effects of PYP on cisplatininduced nephrotoxicity in the HK2 human proximal tubular epithelial cell line. PYP treatment reduced cisplatininduced apoptosis and death of HK2 cells by restoring the Bcell lymphoma2 (Bcl2)associated X protein (Bax)/Bcl2 imbalance, cytochrome c release and caspase3 activation. In addition, PYP activated the redoxsensitive transcription factor NFκB via stimulating the nuclear translocation of p65 in HK2 cells. PYP also restored renal antioxidant levels and increased the total and nuclear accumulation of NF erythroid 2related factor 2 in HK2 cells. PYP markedly attenuated cisplatininduced p38, MAPK and cJun Nterminal kinase phosphorylation. Furthermore, treatment with PYP ameliorated cisplatininduced renal cell damage by upregulating antioxidant defense mechanisms and downregulating the MAPK and NFκB signaling pathways. In addition, mice were divided into three treatment groups (control, cisplatin and PYP + cisplatin) and the effects of PYP were evaluated in a mouse model of cisplatininduced acute kidney injury. The concentrations of blood urea nitrogen and serum creatinine in the PYP + cisplatin group were lower than those in the cisplatin group. The mRNA expression levels of inflammatory factors interleukin6 (IL6), IL1ß, tumor necrosis factorα and monocyte chemoattractant protein1 in the kidney tissues of the PYP + cisplatin group were also lower than those in the cisplatin group. These results suggest that PYP treatment had a preventive effect on nephrotoxicity, specifically by downregulating the MAPK and NFκB signaling pathways and the mRNA levels of inflammatory genes.
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Base de dados:
MEDLINE
Assunto principal:
Extratos Celulares
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Proteínas Quinases p38 Ativadas por Mitógeno
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Injúria Renal Aguda
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Neoplasias
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article