Methods to Study Solo/Orphan Quorum-Sensing Receptors.

Venturi, Vittorio; Subramoni, Sujatha; Sabag-Daigle, Anice; Ahmer, Brian M M

Venturi, Vittorio; Subramoni, Sujatha; Sabag-Daigle, Anice; Ahmer, Brian M M.

Afiliação

Venturi V; International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 34149, Trieste, Italy. venturi@icgeb.org.
Subramoni S; Singapore Centre for Environmental Life Science Engineering, Singapore, Singapore.
Sabag-Daigle A; Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, USA.
Ahmer BMM; Department of Microbial Infection and Immunity, The Ohio State University, Columbus, OH, USA.

Methods Mol Biol ; 1673: 145-159, 2018.

Article em En | MEDLINE | ID: mdl-29130171

RESUMO

LuxR solos/orphans are very widespread among Proteobacteria; however they are surprisingly understudied given that they are likely to play a major role in cell-cell communication in bacteria. Here we describe three simple methodologies/approaches that can be used in order to begin to study this subgroup of quorum sensing-related LuxR receptors.

Assuntos

Proteínas de Bactérias/metabolismo; Biologia Molecular/métodos; Percepção de Quorum; Receptores de Superfície Celular/metabolismo; Simulação por Computador; Ligantes; Proteínas Recombinantes de Fusão/metabolismo; Regulon/genética; Transcrição Gênica

Palavras-chave

E. coli; LuxI; LuxR orphans; LuxR solos; Quorum sensing; Regulon; Salmonella; SdiA; Transcriptional fusion; Transposon

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Receptores de Superfície Celular / Percepção de Quorum / Biologia Molecular Idioma: En Ano de publicação: 2018 Tipo de documento: Article

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