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Clinical Application of Circulating Tumor DNA in the Genetic Analysis of Patients with Advanced GIST.
Xu, Hao; Chen, Liang; Shao, Yang; Zhu, Dongqin; Zhi, Xiaofei; Zhang, Qiang; Li, Fengyuan; Xu, Jianghao; Liu, Xisheng; Xu, Zekuan.
Afiliação
  • Xu H; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Guangzhou Road, Nanjing, Jiangsu Province, China.
  • Chen L; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Guangzhou Road, Nanjing, Jiangsu Province, China.
  • Shao Y; Nanjing Geneseeq Biotechnology Inc., Nanjing, Jiangsu Province.
  • Zhu D; Nanjing Geneseeq Biotechnology Inc., Nanjing, Jiangsu Province.
  • Zhi X; Department of General Surgery, The Affiliated Hospital of Nantong University, Nantong, Jiangsu Province, China.
  • Zhang Q; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Guangzhou Road, Nanjing, Jiangsu Province, China.
  • Li F; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Guangzhou Road, Nanjing, Jiangsu Province, China.
  • Xu J; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Guangzhou Road, Nanjing, Jiangsu Province, China.
  • Liu X; Department of Radiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province.
  • Xu Z; Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Guangzhou Road, Nanjing, Jiangsu Province, China. xuzekuan@njmu.edu.cn.
Mol Cancer Ther ; 17(1): 290-296, 2018 01.
Article em En | MEDLINE | ID: mdl-29133619
Gastrointestinal stromal tumors (GIST) are the most common mesenchymal tumor of digestive tract. In the past, tissue biopsy was the main method for the diagnosis of GISTs. Although, circulating tumor DNA (ctDNA) detection by next-generation sequencing (NGS) may be a feasible and replaceable method for diagnosis of GISTs. We retrospectively analyzed the data for ctDNA and tissue DNA detection from 32 advanced GIST patients. We found that NGS obviously increased the positive rate of ctDNA detection. ctDNA detection identified rare mutations that were not detected in tissue DNA detection. Tumor size and Ki-67 were significant influencing factors of the positive rate of ctDNA detection and concordance between ctDNA and tissue DNA detection. In all patients, the concordance rate between ctDNA and tissue DNA detection was 71.9%, with moderate concordance, but the concordance was strong for patients with tumor size > 10 cm or Ki-67 > 5%. Tumor size, mitotic figure, Ki-67, and ctDNA mutation type were the significant influencing factors of prognosis, but only tumor size and ctDNA mutation type, were the independent prognostic factors for advanced GIST patients. We confirmed that ctDNA detection by NGS is a feasible and promising method for the diagnosis and prognosis of advanced GIST patients. Mol Cancer Ther; 17(1); 290-6. ©2017 AACR.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Tumores do Estroma Gastrointestinal / DNA Tumoral Circulante Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Tumores do Estroma Gastrointestinal / DNA Tumoral Circulante Idioma: En Ano de publicação: 2018 Tipo de documento: Article