New metabolites of hongdenafil, homosildenafil and hydroxyhomosildenafil.
J Pharm Biomed Anal
; 149: 586-590, 2018 Feb 05.
Article
em En
| MEDLINE
| ID: mdl-29197805
ABSTRACT
Recently, illegal sildenafil analogues have emerged, causing serious social issues. In spite of the importance of sildenafil analogues, their metabolic profiles or clinical effects have not been reported yet. In this study, new metabolites of illegal sildenafil analogues such as hongdenafil, homosildenafil, and hydroxyhomosildenafil were determined using liquid chromatography quadrupole-time of flight mass spectrometry (LC-Q-TOF-MS) and tandem mass spectrometry (LC-Q-TOF-MS/MS). To prepare metabolic samples, in vitro and in vivo studies were performed. For in vivo metabolites analysis, urine and feces samples of rats treated with sildenafil analogues were analyzed. For in vitro metabolites analysis, human liver microsomes incubated with sildenafil analogues were extracted and analyzed. All metabolites were characterized by LC-Q-TOF-MS and LC-Q-TOF-MS/MS. As a result, five, six, and seven metabolites were determined in hongdenafil, homosildenafil, and hydroxyhomosildenafil treated samples, respectively. These results could be applied to forensic science and other analytical fields. Moreover, these newly identified metabolites could be used as fundamental data to determine the side effect and toxicity of illegal sildenafil analogues.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Toxicologia Forense
/
Medicamentos Falsificados
/
Inibidores da Fosfodiesterase 5
/
Agentes Urológicos
/
Citrato de Sildenafila
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article