Specific and highly efficient condensation of GC and IC DNA by polyaza pyridinophane derivatives.
Int J Biol Macromol
; 109: 143-151, 2018 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-29247733
ABSTRACT
Two bis-polyaza pyridinophane derivatives and their monomeric reference compounds revealed strong interactions with ds-DNA and RNA. The bis-derivatives show a specific condensation of GC- and IC-DNA, which is almost two orders of magnitude more efficient than the well-known condensation agent spermine. The type of condensed DNA was identified as ψ-DNA, characterized by the exceptionally strong CD signals. At variance to the almost silent AT(U) polynucleotides, these strong CD signals allow the determination of GC-condensates at nanomolar nucleobase concentrations. Detailed thermodynamic characterisation by ITC reveals significant differences between the DNA binding of the bis-derivative compounds (enthalpy driven) and that of spermine and of their monomeric counterparts (entropy driven). Atomic force microscopy confirmed GC-DNA compaction by the bis-derivatives and the formation of toroid- and rod-like structures responsible for the ψ-type pattern in the CD spectra.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Piridonas
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DNA
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Conformação de Ácido Nucleico
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article