[Effect of Myeloma-Derived Exosomes on Surface Activating Receptors of NK Cells].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
; 25(6): 1713-1717, 2017 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-29262903
ABSTRACT
OBJECTIVE:
To investigate the effect of myeloma-derived exosomes on surface activating receptors of NK cells, and to explore the mechanism of the function defect of NK cells.METHODS:
The exosomes from the supernatant of multiple myeloma cell lines RPMI8226 and U266 were extracted by ultracentrifugation, and the size of them was identified under electron microscope; the human primary NK cells were extracted, and were co-cultured with the myeloma-derived exosomes (40 µg/ml), then the expression levels of surface activating receptors NKp46, NKp30 and NKG2D of NK cells at 0,1,4 and 24 hours were detected by flow cytometry.RESULTS:
The exosomes showed small vesicular, sized 30-100 nm under electron microscope. The expression of surface activating receptors of NK cells declined at different degree after co-cultured with myeloma-derived exosomes.CONCLUSION:
Myeloma-derived exosomes can inhibit the expression of surface activating receptors of NK cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Receptores de Superfície Celular
/
Exossomos
/
Receptores de Células Matadoras Naturais
/
Mieloma Múltiplo
Idioma:
Zh
Ano de publicação:
2017
Tipo de documento:
Article