An optimized low-cost protocol for standardized production of iron-free apoferritin nanocages with high protein recovery and suitable conformation for nanotechnological applications.

Moglia, Italo; Santiago, Margarita; Olivera-Nappa, Álvaro; Soler, Mónica

Moglia, Italo; Santiago, Margarita; Olivera-Nappa, Álvaro; Soler, Mónica.

Afiliação

Moglia I; Department of Chemical Engineering, Biotechnology and Materials, FCFM, University of Chile, Beauchef 851, Santiago, Chile.
Santiago M; Center for Biotechnology and Bioengineering - CeBiB, FCFM, University of Chile, Beauchef 851, Santiago, Chile.
Olivera-Nappa Á; Department of Chemical Engineering, Biotechnology and Materials, FCFM, University of Chile, Beauchef 851, Santiago, Chile; Center for Biotechnology and Bioengineering - CeBiB, FCFM, University of Chile, Beauchef 851, Santiago, Chile. Electronic address: aolivera@ing.uchile.cl.
Soler M; Department of Chemical Engineering, Biotechnology and Materials, FCFM, University of Chile, Beauchef 851, Santiago, Chile. Electronic address: msoler@ing.uchile.cl.

J Inorg Biochem ; 183: 184-190, 2018 06.

Article em En | MEDLINE | ID: mdl-29279245

Assuntos

Apoferritinas/química; Nanoestruturas/química; Nanotecnologia/métodos; Cromatografia em Gel; Concentração de Íons de Hidrogênio; Conformação Molecular

Palavras-chave

Apoferritin production; Iron chelators; Iron-devoided ferritin; Monomeric ferritin; Oligomeric ferritin; pH effect

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Apoferritinas / Nanotecnologia / Nanoestruturas Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo

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PubMed Links

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Apoferritinas / Nanotecnologia / Nanoestruturas Idioma: En Ano de publicação: 2018 Tipo de documento: Article