Direct detection of lysine side chain NH3+ in protein-heparin complexes using NMR spectroscopy.
Analyst
; 143(3): 635-638, 2018 Feb 07.
Article
em En
| MEDLINE
| ID: mdl-29292440
ABSTRACT
Two NMR observables, the NζH3+ peak in the HISQC spectrum and Nζ chemical shift difference between the free and heparin-bound forms, can identify binding-interface lysines in protein-heparin complexes. Unlike backbone chemical shifts, these direct probes are stringent and are less prone to either false positives or false negatives.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Heparina
/
Espectroscopia de Ressonância Magnética
/
Lisina
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article