Complete genomic sequence of Raphanus sativus cryptic virus 4 (RsCV4), a novel alphapartitivirus from radish.
Arch Virol
; 163(4): 1097-1100, 2018 Apr.
Article
em En
| MEDLINE
| ID: mdl-29302793
ABSTRACT
The present work reports the discovery and complete genome sequencing of a virus from symptomless radish seedlings, classifiable as a novel member of the genus Alphapartitivirus, family Partitiviridae. Total RNA extracted from germinating seedlings was sequenced using Illumina technology. Bioinformatic analysis of the RNA-seq data revealed two contigs representing the near full-length genomic sequences of two genomic RNAs representing a new virus. Analysis of the genome sequence (excluding the polyA tail, RNA1 1976 nt and RNA2 1751 nt, respectively) showed a genomic organization typical of viruses classed within the Partitiviridae, with each genomic RNA encoding a single open reading frame (ORF). Phylogenetic analysis of the RNA dependent RNA polymerase (RNA1 ORF) and of the capsid protein (RNA2 ORF) clearly showed the new virus can be classified within the genus Alphapartitivirus, but sequence divergence establishes it as a new species, for which the name "Raphanus sativus cryptic virus 4" is proposed.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Filogenia
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Vírus de RNA
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RNA Polimerase Dependente de RNA
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Genoma Viral
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Raphanus
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Proteínas do Capsídeo
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article