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A Comprehensive Protocol Resource for Performing Pooled shRNA and CRISPR Screens.
Cluse, Leonie A; Nikolic, Iva; Knight, Deborah; Madhamshettiwar, Piyush B; Luu, Jennii; Cowley, Karla J; Semple, Timothy; Arnau, Gisela Mir; Shortt, Jake; Johnstone, Ricky W; Simpson, Kaylene J.
Afiliação
  • Cluse LA; Gene Regulation Laboratory, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia.
  • Nikolic I; Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia.
  • Knight D; Gene Regulation Laboratory, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia.
  • Madhamshettiwar PB; Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia.
  • Luu J; Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia.
  • Cowley KJ; Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia.
  • Semple T; Molecular Genomics, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia.
  • Arnau GM; Molecular Genomics, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia.
  • Shortt J; School of Clinical Sciences at Monash Health, Monash University, Clayton, VIC, Australia.
  • Johnstone RW; Gene Regulation Laboratory, Peter MacCallum Cancer Centre, Melbourne, VIC, Australia.
  • Simpson KJ; Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, VIC, Australia.
Methods Mol Biol ; 1725: 201-227, 2018.
Article em En | MEDLINE | ID: mdl-29322420
ABSTRACT
This chapter details a compendium of protocols that collectively enable the reader to perform a pooled shRNA and/or CRISPR screen-with methods to identify and validate positive controls and subsequent hits; establish a viral titer in the cell line of choice; create and screen libraries, sequence strategies, and bioinformatics resources to analyze outcomes. Collectively, this provides an overarching resource from the start to finish of a screening project, making this technology possible in all laboratories.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Biblioteca Gênica / Lentivirus / RNA Interferente Pequeno / Ensaios de Triagem em Larga Escala / Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas Idioma: En Ano de publicação: 2018 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Biblioteca Gênica / Lentivirus / RNA Interferente Pequeno / Ensaios de Triagem em Larga Escala / Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas Idioma: En Ano de publicação: 2018 Tipo de documento: Article