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In vivo biotinylated calpastatin improves the affinity purification of human m-calpain.
Nguyen, Hung Huy; Volkov, Alexander N; Vandenbussche, Guy; Tompa, Peter; Pauwels, Kris.
Afiliação
  • Nguyen HH; VIB - VUB Centre for Structural Biology (VIB CSB), Vlaams Instituut voor Biotechnologie (VIB), Brussels, Belgium; Structural Biology Brussels (SBB), Department of Biotechnology (DBIT), Vrije Universiteit Brussel (VUB), Brussels, Belgium.
  • Volkov AN; VIB - VUB Centre for Structural Biology (VIB CSB), Vlaams Instituut voor Biotechnologie (VIB), Brussels, Belgium; Structural Biology Brussels (SBB), Department of Biotechnology (DBIT), Vrije Universiteit Brussel (VUB), Brussels, Belgium; Jean Jeener NMR Centre, Vrije Universiteit Brussel (VUB), Brus
  • Vandenbussche G; Laboratory for the Structure and Function of Biological Membranes, Centre for Structural Biology and Bioinformatics, Université Libre de Bruxelles (ULB), Brussels, Belgium.
  • Tompa P; VIB - VUB Centre for Structural Biology (VIB CSB), Vlaams Instituut voor Biotechnologie (VIB), Brussels, Belgium; Structural Biology Brussels (SBB), Department of Biotechnology (DBIT), Vrije Universiteit Brussel (VUB), Brussels, Belgium; Institute of Enzymology, Research Centre for Natural Sciences
  • Pauwels K; VIB - VUB Centre for Structural Biology (VIB CSB), Vlaams Instituut voor Biotechnologie (VIB), Brussels, Belgium; Structural Biology Brussels (SBB), Department of Biotechnology (DBIT), Vrije Universiteit Brussel (VUB), Brussels, Belgium. Electronic address: kris.pauwels@vub.be.
Protein Expr Purif ; 145: 77-84, 2018 05.
Article em En | MEDLINE | ID: mdl-29339216
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Calpaína / Cromatografia de Afinidade Idioma: En Ano de publicação: 2018 Tipo de documento: Article
Texto completo
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XML
PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Calpaína / Cromatografia de Afinidade Idioma: En Ano de publicação: 2018 Tipo de documento: Article