MicroRNA expression profiles in benign prostatic hyperplasia.
Mol Med Rep
; 17(3): 3853-3858, 2018 03.
Article
em En
| MEDLINE
| ID: mdl-29359788
ABSTRACT
Although alterations in microRNA (miRNA) expression have been previously investigated prostate cancer, the expression of miRNAs specifically in benign prostate hyperplasia (BPH) of the prostatic stroma remains to be fully elucidated. In the present study, miRNAs and gene expression profiles were investigated using microarray analysis and reverse transcription quantitativepolymerase chain reaction (RTqPCR) in BPH tissue to clarify the associations between miRNA expression and target genes. Prostate tissue samples from five patients with BPH and five healthy men were analyzed using human Affymetrix miRNA and mRNA microarrays and differentially expressed miRNAs were validated using RTqPCR with 30 BPH and 5 healthy control samples. A total of 8 miRNAs, including miRNA (miR)965p, miR12715p, miR213p, miR965p, miR181a5p, miR1433p, miR4428 and miR106a5p were upregulated and 8 miRNAs (miR165p, miR19b5p, miR940, miR25, miR4863p, miR30a3p, let7c and miR191) were downregulated. Additionally, miR965p was demonstrated to have an inhibitory effect on the mRNA expression levels of the following genes Mechanistic target of rapamycin (MTOR), RPTOR independent companion of MTOR complex 2, syntaxin 10, autophagyrelated protein 9A, zinc finger Ebox binding homeobox 1, caspase 2 and protein kinase c ε. Additionally, 16 differentially expressed miRNAs were identified using RTqPCR analysis. This preliminary study provides a solid basis for a further functional study to investigate the underlying regulatory mechanisms of BPH.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Próstata
/
Hiperplasia Prostática
/
RNA Mensageiro
/
Regulação da Expressão Gênica
/
MicroRNAs
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article