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Two approaches for estimating the lower limit of quantitation (LLOQ) of microRNA levels assayed as exploratory biomarkers by RT-qPCR.
Wolfinger, Russell D; Beedanagari, Sudheer; Boitier, Eric; Chen, Tao; Couttet, Philippe; Ellinger-Ziegelbauer, Heidrun; Guillemain, Gregory; Mariet, Claire; Mouritzen, Peter; O'Lone, Raegan; Pine, P Scott; Sharapova, Tatiana; Yan, Jian; Yuen, Peter S; Thompson, Karol L.
Afiliação
  • Wolfinger RD; SAS Institute Inc., Cary, NC, 27513, USA.
  • Beedanagari S; Alkermes Inc., Waltham, MA, 02451, USA.
  • Boitier E; Bristol Myers Squibb, New Brunswick, NJ, USA.
  • Chen T; Sanofi R&D, Disposition Safety and Animal Research, Vitry-sur-Seine, France.
  • Couttet P; Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR, 72079, USA.
  • Ellinger-Ziegelbauer H; Novartis Pharma AG, CH-4057, Basel, CH, Switzerland.
  • Guillemain G; Toxicology, Bayer Pharma AG, 42096, Wuppertal, Germany.
  • Mariet C; Novartis Pharma AG, CH-4057, Basel, CH, Switzerland.
  • Mouritzen P; Sanofi R&D, Disposition Safety and Animal Research, Vitry-sur-Seine, France.
  • O'Lone R; Exiqon, DK-2950, Vedbaek, Denmark.
  • Pine PS; ILSI Health and Environmental Sciences Institute, 1156 15th NW, 2nd Floor, Washington, DC, 20005, USA. rbolone@gmail.com.
  • Sharapova T; National Institute of Standards and Technology, Stanford, CA, 94305, USA.
  • Yan J; AbbVie, Abbott Park, IL, 60064, USA.
  • Yuen PS; Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR, 72079, USA.
  • Thompson KL; NIH/NIDDK, Bethesda, MD, 20892, USA.
BMC Biotechnol ; 18(1): 6, 2018 02 02.
Article em En | MEDLINE | ID: mdl-29391006
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Reação em Cadeia da Polimerase Via Transcriptase Reversa / MicroRNAs / Limite de Detecção Idioma: En Ano de publicação: 2018 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Reação em Cadeia da Polimerase Via Transcriptase Reversa / MicroRNAs / Limite de Detecção Idioma: En Ano de publicação: 2018 Tipo de documento: Article