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Derivation of human induced pluripotent stem cell line (HUSTi001-A) from a 40-year-old patient with idiopathic infertility.
Fang, Fang; Li, Zili; Zhao, Qian; Ye, Zhen; Zhao, Kai; Gu, Xiuli; Shen, Shiliang; Reijo Pera, Renee A; Xiong, Chengliang.
Afiliação
  • Fang F; Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
  • Li Z; Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; Wuhan Tongji Reproductive Medicine Hospital, Wuhan 430013, China.
  • Zhao Q; Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
  • Ye Z; Wuhan Tongji Reproductive Medicine Hospital, Wuhan 430013, China.
  • Zhao K; Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
  • Gu X; Wuhan Tongji Reproductive Medicine Hospital, Wuhan 430013, China.
  • Shen S; Zhong Shen Bioscience Inc., Wuhan, China.
  • Reijo Pera RA; Department of Cell Biology and Neuroscience, Montana State University, Bozeman, MT 59717, USA; Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717, USA.
  • Xiong C; Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; Wuhan Tongji Reproductive Medicine Hospital, Wuhan 430013, China. Electronic address: clxiong951@sina.com.
Stem Cell Res ; 27: 136-139, 2018 03.
Article em En | MEDLINE | ID: mdl-29414604
ABSTRACT
Human fibroblasts were isolated from foreskin of a clinically diagnosed 40-year old patient with idiopathic infertility. The fibroblasts were reprogrammed with the Yamanaka KOSM transcriptional factors using the retroviral vectors. The obtained induced pluripotent stem cell (iPSC) line showed pluripotency verified by the expression of pluripotency markers, NANOG, SOX2, OCT4, TRA-1-60, and SSEA-4. And the iPSC line was demonstrated to have the three germ layers differentiation capacity in vivo by teratoma assay. The iPSC line also showed normal karyotype. This patient-specific iPSC line can be used to explore the mechanism for idiopathic male infertility.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes Induzidas / Infertilidade Idioma: En Ano de publicação: 2018 Tipo de documento: Article
Texto completo
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XML
PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes Induzidas / Infertilidade Idioma: En Ano de publicação: 2018 Tipo de documento: Article