Carbazole alkaloids with antiangiogenic activities from Clausena sanki.
Bioorg Chem
; 77: 387-392, 2018 04.
Article
em En
| MEDLINE
| ID: mdl-29421715
ABSTRACT
Two new carbazole alkaloids 1 and 2, and eleven known congeners 3-13 were isolated and identified from Clausena sanki for the first time. Their structures were elucidated on the basis of extensive UV, IR, MS, NMR spectroscopic data and comparison with literatures. The compounds 1-13 were evaluated by MTT assay to determine whether they decreased VEGF-mediated cell proliferation in HUVECs with Axitinib as positive control. Among them, compounds 1, 2, 6, 8, and 13 (µM) exhibited moderate antiangiogenic activities, which inhibited VEGF-induced HUVEC proliferation in vitro with IC50 values of 12.1 (C.I. 8.2-15.2), 58.1 (C.I. 56.3-63.4), 13.7 (C.I. 9.2-15.4), 16.0 (C.I. 9.5-16.4), and 63.2 (C.I. 57.8-65.7) µM, respectively. Moreover, the antiangiogenic activities of compounds 1-13 were evidenced in vivo in the zebrafish embryo model. As a result, compounds 1, 2, 6, 8, and 13 showed effectively suppress angiogenesis. These research results may guide the search for new natural products with antiangiogenic attributes.
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Base de dados:
MEDLINE
Assunto principal:
Carbazóis
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Inibidores da Angiogênese
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Fatores de Crescimento do Endotélio Vascular
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Alcaloides
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Clausena
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article