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Generation of patient-specific induced pluripotent stem cells from Leber's hereditary optic neuropathy.
Lu, Huai-En; Yang, Yi-Ping; Chen, Yan-Ting; Wu, You-Ren; Wang, Chia-Lin; Tsai, Fu-Ting; Hwang, De-Kuang; Lin, Tai-Chi; Chen, Shih-Jen; Wang, An-Guor; Hsieh, Patrick C H; Chiou, Shih-Hwa.
Afiliação
  • Lu HE; Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan.
  • Yang YP; School of Medicine, National Yang-Ming University, Taipei, Taiwan; Department of Neurosurgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
  • Chen YT; Department of Ophthalmology, Changhua Christian Hospital, Changhua, Taiwan; Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan; Department of Optometry, Central Taiwan University of Science and Technology, Taichung, Taiwan.
  • Wu YR; Institute of Pharmacology, National Yang-Ming University, Taipei, Taiwan.
  • Wang CL; Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan.
  • Tsai FT; Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan.
  • Hwang DK; Department of Ophthalmology, Taipei Veterans General Hospital, Taipei, Taiwan; School of Medicine, National Yang-Ming University, Taipei, Taiwan.
  • Lin TC; Department of Ophthalmology, Taipei Veterans General Hospital, Taipei, Taiwan; Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan.
  • Chen SJ; Department of Ophthalmology, Taipei Veterans General Hospital, Taipei, Taiwan; School of Medicine, National Yang-Ming University, Taipei, Taiwan.
  • Wang AG; Department of Ophthalmology, Taipei Veterans General Hospital, Taipei, Taiwan; School of Medicine, National Yang-Ming University, Taipei, Taiwan.
  • Hsieh PCH; Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
  • Chiou SH; Institute of Pharmacology, National Yang-Ming University, Taipei, Taiwan; Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan; Genomic Research Center, Academia Sinica, Taipei, Taiwan. Electronic address: shchiou@vghtpe.gov.tw.
Stem Cell Res ; 28: 56-60, 2018 04.
Article em En | MEDLINE | ID: mdl-29427840
Leber's hereditary optic neuropathy (LHON) is a maternally inherited mitochondrial disease caused by homoplasmic point mutations in complex I subunit genes of mitochondrial DNA. In this report, we generated an induced pluripotent stem cell (iPSCs) line, TVGH-iPSC-010-09, from the peripheral blood mononuclear cells of a female patient with Leber's hereditary optic neuropathy (LHON) by using the Sendai-virus delivery system. The resulting iPSCs retained the disease-causing mitochondrial DNA mutation, expressed pluripotent markers and could differentiate into the three germ layers. We believe LHON patient-specific iPSCs provide a powerful in vitro model for evaluating the pathological phenotypes of the disease.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas de Cultura de Células / Atrofia Óptica Hereditária de Leber / Células-Tronco Pluripotentes Induzidas Idioma: En Ano de publicação: 2018 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Técnicas de Cultura de Células / Atrofia Óptica Hereditária de Leber / Células-Tronco Pluripotentes Induzidas Idioma: En Ano de publicação: 2018 Tipo de documento: Article