PI3K/AKT signaling pathway involvement in fluoride-induced apoptosis in C2C12â¯cells.
Chemosphere
; 199: 297-302, 2018 May.
Article
em En
| MEDLINE
| ID: mdl-29448197
ABSTRACT
To investigate the mechanisms of fluoride-induced apoptosis, a fluoride-induced C2C12 skeletal muscle cell (C2C12â¯cell) model was established in this study, and the viability of the C2C12â¯cells was measured using an MTT assay. Cell morphological changes were observed via haematoxylin and eosin staining and transmission electron microscopy. Apoptosis was monitored through Hoechst staining. The mRNA and protein expression of PI3K, PDK1, AKT1, BAD, Bcl-2, Bax and caspase-9 were detected through real-time PCR and western blotting, respectively. The results showed that the survival rates of C2C12â¯cells decreased gradually with an increasing fluoride doses. The C2C12â¯cell structure was seriously damaged by fluoride, presenting with pyknosis, mitochondrial ridge disruption and swollen endoplasmic reticulum. Furthermore, the expression of mRNA in PI3K, BAD, Bcl-2, Bax and caspase-9 were significantly increased in the fluoride group (Pâ¯<â¯0.01), while the expression of PDK1 was markedly decreased (Pâ¯<â¯0.01). The expression of protein in BAD, Bcl-2 and Bax were significantly increased in the fluoride group (Pâ¯<â¯0.01), while the expression of PDK1 and P-AKT1 was markedly decreased (Pâ¯<â¯0.01). In conclusion, fluoride-induced apoptosis in C2C12â¯cells is related to the PI3K/AKT signaling pathway.
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Base de dados:
MEDLINE
Assunto principal:
Apoptose
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Músculo Esquelético
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Fosfatidilinositol 3-Quinases
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Proteínas Proto-Oncogênicas c-akt
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Fluoretos
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article