Twin target self-amplification-based DNA machine for highly sensitive detection of cancer-related gene.
Anal Chim Acta
; 1011: 86-93, 2018 Jun 29.
Article
em En
| MEDLINE
| ID: mdl-29475489
ABSTRACT
The sensitive detection of cancer-related genes is of great significance for early diagnosis and treatment of human cancers, and previous isothermal amplification sensing systems were often based on the reuse of target DNA, the amplification of enzymatic products and the accumulation of reporting probes. However, no reporting probes are able to be transformed into target species and in turn initiate the signal of other probes. Herein we reported a simple, isothermal and highly sensitive homogeneous assay system for tumor suppressor p53 gene detection based on a new autonomous DNA machine, where the signaling probe, molecular beacon (MB), was able to execute the function similar to target DNA besides providing the common signal. In the presence of target p53 gene, the operation of DNA machine can be initiated, and cyclical nucleic acid strand-displacement polymerization (CNDP) and nicking/polymerization cyclical amplification (NPCA) occur, during which the MB was opened by target species and cleaved by restriction endonuclease. In turn, the cleaved fragments could activate the next signaling process as target DNA did. According to the functional similarity, the cleaved fragment was called twin target, and the corresponding fashion to amplify the signal was named twin target self-amplification. Utilizing this newly-proposed DNA machine, the target DNA could be detected down to 0.1 pM with a wide dynamic range (6 orders of magnitude) and single-base mismatched targets were discriminated, indicating a very high assay sensitivity and good specificity. In addition, the DNA machine was not only used to screen the p53 gene in complex biological matrix but also was capable of practically detecting genomic DNA p53 extracted from A549â¯cell line. This indicates that the proposed DNA machine holds the potential application in biomedical research and early clinical diagnosis.
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Base de dados:
MEDLINE
Assunto principal:
DNA de Neoplasias
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Proteína Supressora de Tumor p53
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Técnicas de Amplificação de Ácido Nucleico
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article