Spectroscopic analyses and genotoxicity of dioxins in the aquatic environment of Alexandria.
Mar Pollut Bull
; 127: 618-625, 2018 Feb.
Article
em En
| MEDLINE
| ID: mdl-29475705
ABSTRACT
Dioxins have global concerns because of the bioaccumulation tendency and persistency in the environment. Water, seabream Pagrus auratus and seabass Dicentrarchus labrax samples were collected from Abu Qir, Alexandria to evaluate the concentration of dioxin. Fourier Transform Infrared Spectrometer (FTIR) and molecular modeling was applied for elucidating the molecular structure of fish samples. Furthermore, HPLC with UV detection was used to determine the concentration of dioxins (2,8-dichloro dibenzo-p-dioxin). RT-PCR assay was conducted to verify the expression of some immune genes in the fish species as a result of water pollution. The average detected concentrations varied from 0.2 to 1.3µg/l. Gene expression revealed that MHC class 1 and C3 were highly upregulated in liver and muscle of seabass and seabream while T2BP was highly regulated in seabass liver and seabream muscle and seabass muscle for transferrin, FTIR and molecular modeling indicate that dioxin finds its way to fish protein.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Poluentes Químicos da Água
/
Bass
/
Dano ao DNA
/
Monitoramento Ambiental
/
Dourada
/
Dioxinas
/
Mutagênicos
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article