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Proteome analysis reveals that de novo regenerated mucosa over fibula flap-reconstructed mandibles resembles mature keratinized oral mucosa.
Kumar, Vinay V; James, Bonney L; Ruß, Manuela; Mikkat, Stefan; Suresh, Amritha; Kämmerer, Peer W; Glocker, Michael O.
Afiliação
  • Kumar VV; Integrated Head and Neck Oncology Research Program, Mazumdar Shaw Centre for Translational Research, Mazumdar Shaw Medical Foundation, Bommasandra Indl Area, Bangalore, India; Department of Head and Neck Surgery, Mazumdar Shaw Medical Center, Narayana Health, Bommasandra, Bengaluru, India; Departmen
  • James BL; Integrated Head and Neck Oncology Research Program, Mazumdar Shaw Centre for Translational Research, Mazumdar Shaw Medical Foundation, Bommasandra Indl Area, Bangalore, India.
  • Ruß M; Proteome Center Rostock, University Medicine Rostock, Rostock, Germany.
  • Mikkat S; Proteome Center Rostock, University Medicine Rostock, Rostock, Germany; Core Facility Proteome Analysis, University Medicine Rostock, Rostock, Germany.
  • Suresh A; Integrated Head and Neck Oncology Research Program, Mazumdar Shaw Centre for Translational Research, Mazumdar Shaw Medical Foundation, Bommasandra Indl Area, Bangalore, India.
  • Kämmerer PW; Department of Oral Maxillofacial and Plastic Surgery, University Medicine Rostock, Rostock, Germany.
  • Glocker MO; Proteome Center Rostock, University Medicine Rostock, Rostock, Germany. Electronic address: http://www.pzr.med.uni-rostock.de.
Oral Oncol ; 78: 207-215, 2018 03.
Article em En | MEDLINE | ID: mdl-29496052
AIM: The aim of this study was to determine whether intra-oral de novo regenerated mucosa (D) that grew over free fibula flap reconstructed-mandibles resembled the donor tissue i.e. external skin (S) of the lateral leg, or the recipient site tissue, i.e. keratinized oral mucosa (K). MATERIALS AND METHODS: Differential proteome analysis was performed with ten tissue samples from each of the three groups: de novo regenerated mucosa (D), external skin (S), and keratinized oral mucosa (K). Expression differences of cornulin and involucrin were validated by Western blot analysis and their spatial distributions in the respective tissues were ascertained by immunohistochemistry. RESULTS: From all three investigated tissue types a total of 1188 proteins were identified, 930 of which were reproducibly and robustly quantified by proteome analysis. The best differentiating proteins were assembled in an oral mucosa proteome signature that encompasses 56 differentially expressed proteins. Principal component analysis of both, the 930 quantifiable proteins and the 56 oral mucosa signature proteins revealed that the de novo regenerated mucosa resembles keratinized oral mucosa much closer than extra-oral skin. Differentially expressed cornification-related proteins comprise proteins from all subclasses of the cornified cell envelope. Prominently expressed in intra-oral mucosa tissues were (i) cornifin-A, cornifin-B, SPRR3, and involucrin from the cornified-cell-envelope precursor group, (ii) S100A9, S100A8 and S100A2 from the S100 group, and (iii) cornulin which belongs to the fused-gene-protein group. CONCLUSION: According to its proteome signature de novo regenerated mucosa over the free fibula flap not only presents a passive structural surface layer but has adopted active tissue function.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteoma / Retalhos de Tecido Biológico / Fíbula / Queratinas / Mandíbula / Mucosa Bucal Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteoma / Retalhos de Tecido Biológico / Fíbula / Queratinas / Mandíbula / Mucosa Bucal Idioma: En Ano de publicação: 2018 Tipo de documento: Article