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Response of digestive enzymes and esterases of ecotoxicological concern in earthworms exposed to chlorpyrifos-treated soils.
Sanchez-Hernandez, Juan C; Ríos, Juan Manuel; Attademo, Andrés M.
Afiliação
  • Sanchez-Hernandez JC; Laboratory of Ecotoxicology, Faculty of Environmental Science and Biochemistry, University of Castilla-La Mancha, 45071, Toledo, Spain. juancarlos.sanchez@uclm.es.
  • Ríos JM; Laboratorio de Química Ambiental, Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales (IANIGLA)-CONICET, P.O. Box 131, ZC5500, Mendoza, Argentina.
  • Attademo AM; Laboratorio de Ecotoxicología, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral (FBCB-UNL-CONICET), Paraje El Pozo s/n, 3000, Santa Fe, Argentina.
Ecotoxicology ; 27(7): 890-899, 2018 Sep.
Article em En | MEDLINE | ID: mdl-29497918
Assessment of organophosphorus (OP) pesticide exposure in non-target organisms rarely involves non-neural molecular targets. Here we performed a 30-d microcosm experiment with Lumbricus terrestris to determine whether the activity of digestive enzymes (phosphatase, ß-glucosidase, carboxylesterase and lipase) was sensitive to chlorpyrifos (5 mg kg-1 wet soil). Likewise, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities were measured in the wall muscle and gastrointestinal tissues as indicators of OP exposure. Chlorpyrifos inhibited the acid phosphatase (34% of controls), carboxylesterase (25.6%) and lipase activities (31%) in the gastrointestinal content. However, in the gastrointestinal tissue, only the carboxylesterase and lipase activities were significantly depressed (42-67% carboxylesterase inhibition in the foregut and crop/gizzard, and 15% lipase inhibition in the foregut). Chlorpyrifos inhibited the activity of both cholinesterases in the gastrointestinal tissues, whereas the AChE activity was affected in the wall muscle. These results suggested chlorpyrifos was widely distributed throughout the earthworm body after 30 d of incubation. Interestingly, we found muscle carboxylesterase activity strongly inhibited (92% of control) compared with that detected in the gastrointestinal tissues of the same OP-exposed individuals. This finding was explained by the occurrence of pesticide-resistant esterases in the gastrointestinal tissues, which were evidenced by zymography. Our results suggest that digestive processes of L. terrestris may be altered by chlorpyrifos, as a consequence of the inhibitory action of the insecticide on some digestive enzymes.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oligoquetos / Poluentes do Solo / Clorpirifos / Inseticidas Idioma: En Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oligoquetos / Poluentes do Solo / Clorpirifos / Inseticidas Idioma: En Ano de publicação: 2018 Tipo de documento: Article