Construction of a ceRNA network reveals potential lncRNA biomarkers in rectal adenocarcinoma.
Oncol Rep
; 39(5): 2101-2113, 2018 May.
Article
em En
| MEDLINE
| ID: mdl-29512732
ABSTRACT
Competing endogenous RNAs (ceRNAs) render the functions of long noncoding RNAs (lncRNAs) more complicated during cancer processes. Potential lncRNA biomarkers and their roles as ceRNAs have not been clearly described for rectal adenocarcinoma (READ). In the present study, we extracted data from The Cancer Genome Atlas (TCGA) including data from 167 tumor samples and 10 adjacent nontumor samples. A total of 202 lncRNAs, 190 microRNAs (miRNAs) and 1,530 mRNAs were identified as READspecific RNAs [log2(foldchange)>2, FDR<0.01]. The Gene Ontology (GO) biological processes and the Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathways were analysed for 1,530 specific mRNAs. Among 202 READspecific lncRNAs, 7 lncRNAs were identified as being associated with overall survival of READ patients. Then, a ceRNA network was constructed with 34 key lncRNAs, 25 miRNAs and 65 mRNAs. A total of 7 lncRNAs from the network were revealed to be linked to clinical features. The results of qRTPCR ascertained that our analysis was credible. Overall, this research provides a novel perspective from which to study the lncRNArelated ceRNA network in READ and assists in the identification of new potential biomarkers to be used for diagnostic and prognostic purposes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Retais
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RNA Mensageiro
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Biologia Computacional
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MicroRNAs
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Redes Reguladoras de Genes
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RNA Longo não Codificante
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article