Mini G protein probes for active G protein-coupled receptors (GPCRs) in live cells.

Wan, Qingwen; Okashah, Najeah; Inoue, Asuka; Nehmé, Rony; Carpenter, Byron; Tate, Christopher G; Lambert, Nevin A

Wan, Qingwen; Okashah, Najeah; Inoue, Asuka; Nehmé, Rony; Carpenter, Byron; Tate, Christopher G; Lambert, Nevin A.

Afiliação

Wan Q; Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta University, Augusta, Georgia 30912.
Okashah N; Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta University, Augusta, Georgia 30912.
Inoue A; Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi 980-8578 Japan.
Nehmé R; MRC Laboratory of Molecular Biology, Cambridge CB20QH, United Kingdom.
Carpenter B; MRC Laboratory of Molecular Biology, Cambridge CB20QH, United Kingdom.
Tate CG; MRC Laboratory of Molecular Biology, Cambridge CB20QH, United Kingdom.
Lambert NA; Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta University, Augusta, Georgia 30912. Electronic address: nelambert@augusta.edu.

J Biol Chem ; 293(19): 7466-7473, 2018 05 11.

Article em En | MEDLINE | ID: mdl-29523687

Assuntos

Proteínas de Ligação ao GTP/metabolismo; Sondas Moleculares/metabolismo; Receptores Acoplados a Proteínas G/metabolismo; Sítios de Ligação; Compartimento Celular; Transferência de Energia; Células HEK293; Humanos; Luciferases/metabolismo; Microscopia Confocal; Mutação; Ligação Proteica; Receptores Acoplados a Proteínas G/genética

Palavras-chave

BRET; G protein; G proteincoupled receptor (GPCR); NanoLuc; arrestin; biosensor; mini G protein; molecular pharmacology; protein complementation

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sondas Moleculares / Proteínas de Ligação ao GTP / Receptores Acoplados a Proteínas G Idioma: En Ano de publicação: 2018 Tipo de documento: Article

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