Whole-Genome Bisulfite Sequencing for the Analysis of Genome-Wide DNA Methylation and Hydroxymethylation Patterns at Single-Nucleotide Resolution.

Kernaleguen, Magali; Daviaud, Christian; Shen, Yimin; Bonnet, Eric; Renault, Victor; Deleuze, Jean-François; Mauger, Florence; Tost, Jörg

Whole-Genome Bisulfite Sequencing for the Analysis of Genome-Wide DNA Methylation and Hydroxymethylation Patterns at Single-Nucleotide Resolution.

Kernaleguen, Magali; Daviaud, Christian; Shen, Yimin; Bonnet, Eric; Renault, Victor; Deleuze, Jean-François; Mauger, Florence; Tost, Jörg.

Afiliação

Kernaleguen M; Laboratory for Epigenetics and Environment, Centre National de Recherche en Génomique Humaine, CEA-Institut de Biologie Francois Jacob, Evry, France.
Daviaud C; Laboratory for Epigenetics and Environment, Centre National de Recherche en Génomique Humaine, CEA-Institut de Biologie Francois Jacob, Evry, France.
Shen Y; Laboratory for Epigenetics and Environment, Centre National de Recherche en Génomique Humaine, CEA-Institut de Biologie Francois Jacob, Evry, France.
Bonnet E; Laboratory for Bio-analysis, Centre National de Recherche en Génomique Humaine, CEA-Institut de Biologie Francois Jacob, Evry, France.
Renault V; Laboratory for Bioinformatics, Fondation Jean Dausset - CEPH, Paris, France.
Deleuze JF; Laboratory for Epigenetics and Environment, Centre National de Recherche en Génomique Humaine, CEA-Institut de Biologie Francois Jacob, Evry, France.
Mauger F; Laboratory for Bioinformatics, Fondation Jean Dausset - CEPH, Paris, France.
Tost J; Laboratory for Epigenetics and Environment, Centre National de Recherche en Génomique Humaine, CEA-Institut de Biologie Francois Jacob, Evry, France.

Methods Mol Biol ; 1767: 311-349, 2018.

Article em En | MEDLINE | ID: mdl-29524144

Assuntos

Metilação de DNA; DNA/genética; Sequenciamento Completo do Genoma/métodos; DNA/análise; Epigênese Genética; Genoma Humano; Genômica/métodos; Humanos; Software; Sulfitos/química; Fluxo de Trabalho

Palavras-chave

Bisulfite conversion; DNA methylation; Hydroxymethylation; Low input; Ovation® Methyl-seq; Oxidative bisulfite sequencing; PBAT; Spike-in; TrueMethyl® WholeGenome; Whole-genome bisulfite sequencing

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: DNA / Metilação de DNA / Sequenciamento Completo do Genoma Idioma: En Ano de publicação: 2018 Tipo de documento: Article

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