Synchrotron X-Ray Diffraction Dynamic Sampling for Protein Crystal Centering.
IS&T Int Symp Electron Imaging
; 2017: 6-9, 2017.
Article
em En
| MEDLINE
| ID: mdl-29527589
ABSTRACT
A supervised learning approach for dynamic sampling (SLADS) was developed to reduce X-ray exposure prior to data collection in protein structure determination. Implementation of this algorithm allowed reduction of the X-ray dose to the central core of the crystal by up to 20-fold compared to current raster scanning approaches. This dose reduction corresponds directly to a reduction on X-ray damage to the protein crystals prior to data collection for structure determination. Implementation at a beamline at Argonne National Laboratory suggests promise for the use of the SLADS approach to aid in the analysis of X-ray labile crystals. The potential benefits match a growing need for improvements in automated approaches for microcrystal positioning.
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MEDLINE
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En
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2017
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Article