Modeling Environmentally-Induced Motor Neuron Degeneration in Zebrafish.
Sci Rep
; 8(1): 4890, 2018 03 20.
Article
em En
| MEDLINE
| ID: mdl-29559645
ABSTRACT
Zebrafish have been used to investigate motor neuron degeneration, including as a model system to examine the pathogenesis of amyotrophic lateral sclerosis (ALS). The use of zebrafish for this purpose has some advantages over other in vivo model systems. In the current paper, we show that bisphenol A (BPA) exposure in zebrafish embryos results in motor neuron degeneration with affected motor function, reduced motor axon length and branching, reduced neuromuscular junction integrity, motor neuron cell death and the presence of activated microglia. In zebrafish, motor axon length is the conventional method for estimating motor neuron degeneration, yet this measurement has not been confirmed as a valid surrogate marker. We also show that reduced motor axon length as measured from the sagittal plane is correlated with increased motor neuron cell death. Our preliminary timeline studies suggest that axonopathy precedes motor cell death. This outcome may have implications for early phase treatments of motor neuron degeneration.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Doença dos Neurônios Motores
/
Degeneração Neural
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article