UFH-001 cells: A novel triple negative, CAIX-positive, human breast cancer model system.
Cancer Biol Ther
; 19(7): 598-608, 2018 07 03.
Article
em En
| MEDLINE
| ID: mdl-29561695
ABSTRACT
Human cell lines are an important resource for research, and are often used as in vitro models of human diseases. In response to the mandate that all cells should be authenticated, we discovered that the MDA-MB-231 cells that were in use in our lab, did not validate based on the alleles of 9 different markers (STR Profile). We had been using this line as a model of triple negative breast cancer (TNBC) that has the ability to form tumors in immuno-compromised mice. Based on marker analysis, these cells most closely resembled the MCF10A line, which are a near diploid and normal mammary epithelial line. Yet, the original cells express carbonic anhydrase IX (CAIX) both constitutively and in response to hypoxia and are features that likely drive the aggressive nature of these cells. Thus, we sought to sub-purify CAIX-expressing cells using Fluorescence Activated Cell Sorting (FACS). These studies have revealed a new line of cells that we have name UFH-001, which have the TNBC phenotype, are positive for CAIX expression, both constitutively and in response to hypoxia, and behave aggressively in vivo. These cells may be useful for exploring mechanisms that underlie progression, migration, and metastasis of this phenotype. In addition, constitutive expression of CAIX allows its evaluation as a therapeutic target, both in vivo and in vitro.
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MEDLINE
Assunto principal:
Biomarcadores Tumorais
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Linhagem Celular Tumoral
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Neoplasias de Mama Triplo Negativas
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Anidrase Carbônica IX
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Antígenos de Neoplasias
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article