Metabolic control of cell fate bifurcations in a hematopoietic progenitor population.
Immunol Cell Biol
; 96(8): 863-871, 2018 09.
Article
em En
| MEDLINE
| ID: mdl-29570858
ABSTRACT
Growth signals drive hematopoietic progenitor cells to proliferate and branch into divergent cell fates, but how unequal outcomes arise from a common progenitor is not fully understood. We used steady-state analysis of in vivo hematopoiesis and Fms-related tyrosine kinase 3 ligand (Flt3L)-induced in vitro differentiation of dendritic cells (DCs) to determine how growth signals regulate lineage bias. We found that Flt3L signaling induced anabolic activation and proliferation of DC progenitors, which was associated with DC differentiation. Perturbation of processes associated with quiescence and catabolism, including AMP-activated protein kinase signaling, fatty acid oxidation, or mitochondrial clearance increased development of cDC2 cells at the expense of cDC1 cells. Conversely, scavenging anabolism-associated reactive oxygen species skewed differentiation toward cDC1 cells. Sibling daughter cells of dividing DC progenitors exhibited unequal expression of the transcription factor interferon regulatory factor 8, which correlated with clonal divergence in FoxO3a signaling and population-level bifurcation of cell fate. We propose that unequal transmission of growth signals during cell division might support fate branches during proliferative expansion of progenitors.
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MEDLINE
Assunto principal:
Células Dendríticas
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Células-Tronco Hematopoéticas
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Fatores Reguladores de Interferon
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Ácidos Graxos
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Proteínas de Membrana
Idioma:
En
Ano de publicação:
2018
Tipo de documento:
Article